Vitamin D + Allostatic Load (DAL+)

The D+ research group was established to understand the relationship between vitamin D and selected dietary supplements (+), and the modern Allostatic Load (AL) model hypothesis. Therefore, the DAL+ team aims to study the physiological and pathological responses associated with therapeutic protocols involving medications, dietary supplements, and functional beverages, including both widely available and newer products. Monitoring early changes in the AL model is a keystone of the research group’s philosophy for understanding modern individual physiological changes in health and disease.

The research group focuses on evaluating therapeutic dosages, individual consumption patterns, and product-specific characteristics as key quantitative and qualitative parameters. These factors are investigated as potential early indicators of changes within the AL model and their association with individual health status. 

The team prioritizes the study of widely consumed nutritional supplements within local and Arab populations, particularly vitamins and minerals, as well as commonly consumed and emerging beverages. Specific research topics include vitamin D, omega-3 fatty acids, energy drinks, and stimulant-containing beverages such as coffee and tea. 

DAL+ studies involve the extraction, quantification, and characterization of bioactive compounds found in beverages and plants. This includes identifying active ingredients, assessing their bioavailability, evaluating their biological activity, and determining their potential health effects. The DAL+ research team links research data and findings with bioinformative analysis to understand targeted changes.

The group aims to integrate compositional analysis of bioactive compounds with functional and clinical assessments, linking intake patterns to physiological and pathological changes in individuals. Particular emphasis is placed on prevalent health conditions, including cardiovascular diseases, diabetes mellitus, and neurological disorders.

Allostatic Load changes and neuroendocrine associations: Early prediction associated with recent life style patterns; Stress , obesity , sleep deprivation , dose – dependent supplementation , and functional beverages, including both widely consumed and newly introduced products.

Recent High-Impact (2025–2026):

​1. The Potential Effect of Vitamin D Supplement on Selected Coagulability Predictors in Vape-Exposed Female Rats . Aman M. Hammad, Mahmoud Abu Samak, Rana Abu Farha, Lujain F. Alzaghari, Abdelrahim Alqudah, Diana Malaeb, Khaldoun Rasem Shnewer, Souheil Hallit, Muna Barakat . bioRxiv 2026.02.25.708056;

doi: https://doi.org/10.64898/2026.02.25.708056

.

2. Al-Zoubi, Sojoud M.; Awwad, Shady H.; Issa, Reem A.; Daraosheh, Ahmad Q.; Al-Qaisi, Talal; Farah, Husni; Omari, Khaled W.; Mohammad, Beisan A.; Amer, Mumen; Khuzaie, Rula F.; and Abu-Samak, Mahmoud S. (2025) ” Quantification of Bioactive Compounds in Coffea Arabica and Their Impact with Omega-3 Supplementation on Non-High-Density Lipoprotein Levels in Hyperlipidemia-Induced Rats,” Baghdad Science Journal: Vol. 22: Iss. 8, Article 11.

DOI: https://doi.org/10.21123/2411-7986.5022

.

3. The Antimicrobial Activity of Combination of Vitamin D3 and Omega-3 against Pathogenic Microorganisms.Authors: Mohammad A. A. AL Najjar1, Shaymaa B. Abdulrazzaq1, Shatha Al-Shaer2, Muna Barakat3, Amin Omar1, Rafat Zrieq4, Eliza Hasen2 and Mahmoud Abu Samak3 . Combinatorial Chemistry & High Throughput Screening, Volume 28, Issue 8, May 2025, p. 1278 – 1289.DOI: https://doi.org/10.2174/0113862073293834240425061327

.

4. Beisan A. MOHAMMAD, Ghaleb Ali ORIQUAT, Heba A Khader, Ahmad R. ALSAYED, Husni Shukri FARAH, Jehad F. ALHMOUD, Mohammad A. A AL-NAJJAR, Luai HASOUN, Shaimaa M. Abdelhalim, Shady H. Awwad, Mahmoud S. Abu-Samak  https://pharmacypractice.org/index.php/pp/article/view/3050

.

5. Sadah AHA, Issa R, Farah H, Oriquat G, Awwad SH, Mosleh I, Mohammad B A, Aljaberi A, Al-Najjar M, Abu-Samak MS. Effect of eicosapentaenoic acid, docosahexaenoic acid, and their combination on selected atherogenic biomarkers in a high-fat diet rat model. J Appl Pharm Sci. 2025;15(03):150–159. http://doi.org/10.7324/JAPS.2025.196085

.

6. The effect of vitamin D3 and omega-3 combination, taken orally, on triglycerides, lining of intestine, and the biodiversity of gut microbiota in healthy rats

SB Abdulrazzaq, M Abu-Samak, A Omar, M Barakat… – Journal of Applied Microbiology, 2024. 

.

7. Rafeef AL Khaled, Jehad F. Alhmoud, Reem Issa, Heba A. Khader, Beisan A. Mohammad, Ahmad R. Alsayed, Khalid Abu Khadra, Maha Habash, Ahmad Aljaberi, Luai Hasoun, Mahmoud S. Abu-Samak, Ibrahim mosleh

6602024 May 31 [cited 2026 May 4];22(2):1-10. https://pharmacypractice.org/index.php/pp/article/view/2951​

.

8. Sara M Alimam, Jehad F Alhmoud, Heba A Khader, Ahmad R Alsayed, Mohammad Abusamak, Beisan A Mohammad, Ibrahim Mosleh, Khalid Abu Khadra, Ahmad Aljaberi, Maha Habash, Luai Hasoun, Mahmoud S Abu-Samak. 2024. Effect of weekly high-dose vitamin D3 supplementation on the association between circulatory FGF-23 and A1c levels in people with vitamin D deficiency: A randomized controlled J Appl Pharm Sci. 2024;22(03.Authors ​.